Dethiobiotin synthase

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August undefined, 2024

In enzymology, a dethiobiotin synthase (EC 6.3.3.3) is an enzyme that catalyzes the chemical reaction ATP + 7,8-diaminononanoate + CO2 ADP + phosphate + dethiobiotin The 3 substrates of this enzyme are ATP, 7,8-diaminononanoate, and CO2, whereas its 3 products are ADP, phosphate, and dethiobiotin. WebEC Tree. 6 Ligases. 6.3 Forming carbon-nitrogen bonds. 6.3.3 Cyclo-ligases. 6.3.3.3 dethiobiotin synthase. IUBMB Comments. CTP has half the activity of ATP. Specify your search results. Mark a special word or phrase in this record:

Biotin, a universal and essential cofactor: synthesis, ligation and

WebMar 9, 2024 · biotin synthase. GeneRIFs: Gene References Into Functions. In biotin synthase, abstraction of a hydrogen atom from the C9 methyl group of dethiobiotin results in formation of a dethiobiotinyl methylene carbon radical, which is quenched by a sulfur atom to form a new carbon-sulfur bond in 9-mercaptodethiobiotin. WebJan 11, 2024 · BioD, dethiobiotin synthase In contrast to the preceding enzymes (with the exception of BioU), BioD (DTB synthase) catalyzes an unusually interesting step. This is the mechanistically unusual reaction of ATP (or CTP) dependent insertion of CO 2 between the N7 and N8 nitrogen atoms of DAPA to form the ureido moiety of biotin. highclere gardens banbury

M-CSA Mechanism and Catalytic Site Atlas

Webd-Desthiobiotin; d-Desthiobiotin is used in affinity chromatography and protein chromatography. d-Desthiobiotin can be used for protein labeling, detection and isolation. WebJan 13, 1994 · Strands 1, 4, 5, and 6 form the classic mononucleotide-binding motif as found in other ATP- and GTP-binding proteins. Dethiobiotin synthase from E. coli is a dimer built up of two identical subunits. The dimer is formed through packing along a molecular two-fold axis, which in the crystals coincides with the crystallographic two-fold axis. WebOct 15, 1998 · The crystal structure of the 224-residue protein dethiobiotin synthetase from Escherichia coli has been refined using X-ray diffraction data at 0.97 A resolution at 100 K. The model, consisting of 4143 protein atoms including 1859 H atoms and 436 solvent sites, was refined to a final R factor of 11.6% for all reflections, and has an estimated ... how far is walland tn from pigeon forge tn

Cloning, expression and characterization of histidine-tagged biotin ...

Reductive Cleavage of S-Adenosylmethionine by Biotin Synthase …

WebMycobacterium tuberculosis dethiobiotin synthase (MtDTBS) is a crucial enzyme involved in the biosynthesis of biotin in the causative agent of tuberculosis, M. tuberculosis. … WebMay 1, 2016 · The function of biotin synthase is to convert dethiobiotin, a product of dethiobiotin synthase (encoded by bioD), the third enzyme in the biotin biosynthesis pathway, to biotin. Biotin is an indispensable nutrient found in all living cells. Microorganisms, plants and a few fungi (most fungi obtain it from the environment) are … highclere gardens plymouthWebDethiobiotin synthetase Methods Enzymol. 1979;62:348-52. doi: 10.1016/0076-6879 (79)62241-3. Authors M A Eisenberg , K Krell PMID: 374977 DOI: 10.1016/0076-6879 … highclere gift shop

"WebDec 14, 2024 · Mycobacterium tuberculosis dethiobiotin synthase ( Mt DTBS) is a crucial enzyme involved in the biosynthesis of biotin in the causative agent of tuberculosis, M. … " - Dethiobiotin synthase

Dethiobiotin synthase

RCSB PDB - 7L1J: Mycobacterium tuberculosis dethiobiotin …

WebMar 28, 1995 · One of these enzymes, ATP-dependent dethiobiotin synthetase, catalyzes the carboxylation of 7,8-diaminopelargonic acid leading to the formation of the ureido ring of biotin. The enzyme belongs to the class of ATP-dependent carboxylases and we present here the first crystal structure determined for this class of enzyme. WebBiotin synthase (BioB) catalyzes the insertion of a sulfur atom between the C6 and C9 carbons of dethiobiotin. Reconstituted BioB from Escherichia coli contains a [4Fe-4S]2+/1+ cluster thought to be involved in the reduction and cleavage of S-adenosylmethionine (AdoMet), generating methionine and the reactive 5′-deoxyadenosyl radical responsible …

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WebBiotin synthase (BioB) catalyzes the oxidative insertion of a sulfur atom between the C6 methylene and the C9 methyl positions in dethiobiotin. The enzyme couples oxidation … Webdethiobiotin synthase. DTB synthetase. (7R,8S)-7,8-diammoniononanoate + ATP + CO2 <=> (4R,5S)-dethiobiotin + ADP + 3 H (+) + phosphate. CTP has half the activity of …

WebNov 1, 2012 · Biotin synthase is an S-adenosyl-l-methionine (SAM or AdoMet) radical enzyme that catalyzes reduction of the AdoMet sulfonium to produce 5′-deoxyadenosyl radicals, high-energy carbon radicals that can directly … WebJan 11, 2024 · Biotin synthase belongs to the radical SAM superfamily and contains the signature Cys-X-X-X-Cys-X-X-Cys motif sequence required for [4Fe-4S] cluster binding. …

WebJan 2, 2004 · The crystal structure of biotin synthase from Escherichia coli in complex with S-adenosyl-L-methionine and dethiobiotin has been determined to 3.4 angstrom resolution.This structure addresses how “AdoMet radical” or “radical SAM” enzymes use Fe 4 S 4 clusters and S-adenosyl-L-methionine to generate organic radicals.Biotin synthase …

WebBiotin synthase catalyzes formation of the thiophane ring through stepwise substitution of a sulfur atom for hydrogen atoms at the C9 and C6 positions of dethiobiotin. Biotin synthase is a radical S-adenosylmethionine (SAM) enzyme that reductively cleaves S-adenosylmethionine, generating 5′-deoxyadenosyl radicals that initially abstract a …

WebDethiobiotin synthase is involved in biotin biosynthesis and catalyses the reaction (CO2 + 7,8-diaminononanoate + ATP = dethiobiotin + phosphate + ADP). The enzyme binds ATP (see motif in first 12 residues of the SEED alignment) and requires magnesium as a co-factor. [Biosynthesis of cofactors, prosthetic groups, and carriers, Biotin] how far is walland tn from meWebSep 5, 1995 · Dethiobiotin Synthetase: The Carbonylation of 7,8-Diaminononanoic Acid Proceeds Regiospecifically via the N7-Carbamate. Biochemistry 1995, 34 (35) , 10976-10984. highclere game fairWebdethiobiotin synthetase, dethiobiotin synthase, more top print hide 6 entries Go to Reaction Search REACTION REACTION DIAGRAM COMMENTARY ORGANISM … highclere grampusWebJun 13, 2005 · Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism. Gene Name bioB Uniprot … highclere groundsWebBiotin synthase catalyzes formation of the thiophane ring through stepwise substitution of a sulfur atom for hydrogen atoms at the C9 and C6 positions of dethiobiotin. Biotin … how far is wallaceburg from windsor ontarioWebBiotin synthase, a member of the ‘radical SAM’ (S-adenosylmethionine) family, converts DTB (dethiobiotin) into biotin. The active form of the Escherichia coli enzyme contains two (Fe-S ... highclere hall bournemouthWebApr 7, 1999 · Biotin synthase, the enzyme which catalyses the conversion of dethiobiotin (DTB) to biotin has an absolute requirement for S-adenosylmethionine (AdoMet) which is … how far is wallaroo from adelaide