Phenotypes of wehi 231
WebSep 1, 1994 · These signals involve a series of metabolic events including Several reports have established that the murine WEHI-231 the rapid induction of protein tyrosine phosphorylation B lymphoma has the surface phenotype of an immature mediated by several protein tyrosine kinases (PTK): B cell: mIgM+, mIgD-, FcRIOW MHC class … Webma. The murine lymphoma line WEHI 231 has been charac-terized as an early B cell; it bears surface-bound IgM and has unrearranged c-myc genes. Following treatment of a WEHI …
Phenotypes of wehi 231
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WebWEHI-231 is a B lymphocyte cell that was isolated from a mouse. This cell line was deposited by NL Warner and L Lanier. WEHI-231 can be used in immunology research. …
WebMay 1, 2004 · B cell receptor (BCR) engagement of murine WEHI 231 immature B lymphoma cells leads sequentially to a drop in NF-κB and c-Myc, and induction of the p27 Kip1 cyclin-dependent kinase inhibitor, which promotes growth arrest and apoptosis. BCR engagement was recently shown to induce a drop in phosphatidylinositol 3-kinase (PI3K)/Akt signaling, … WebNov 7, 2014 · To determine percent infectivity, cells were stained for the MuNoV protease-RdRp (ProPol) nonstructural proteins. Although 80 to 90% of RAW264.7 and WEHI-231 cells were productively infected, only 5 to 15% of M12 cells were productively infected (Fig. 1C and fig. S2).To determine whether M12 cultures cleared this low-level infection or instead …
WebCD24 is a two-chain glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed at multiple stages of B-cell development, beginning with the bone marrow pro-B-cell compartment and continuing through mature, surface Ig positive B-cells. Plasma cell expression is very low or negative. WebDoi T, Motoyama N, Tokunaga A, Watanabe T. Death signals from theBcell antigen receptor target mitochondria, activating necrotic and apoptotic death cascades in a murine B cell line, WEHI-231. Int Immunol 1999; 11: 933–941. Article PubMed Google Scholar Bras A, Ruiz-Vela A, Gonzalez de Buitrago G, Martinez-AC.
WebDec 4, 2014 · WEHI-231 cells transduced with an expression vector encoding FLAG-tagged A1 served as a positive control. ( c) For immunoprecipitation assays, 5 × 10 6 mouse WEHI-231 B lymphoma cells were...
WebJan 12, 2007 · Here, we describe the non-redundant roles of caspase-activated DNase (CAD) and DNaseγ during apoptosis in the immature B-cell line WEHI-231. These cells induce DNA-ladder formation and nuclear ... firebase tomahawk movieWeb3.3. Low Levels of Hg 2+ Attenuate Phosphorylation of ERK, Accentuate Phosphorylation of Syk and BLNK, but Has Little Effect on Phosphorylation of BTK during BCR Mediated Signal Transduction in WEHI-231 Cells. We next investigated the effect of Hg 2+ on BCR signaling upstream of ERK. BCR signaling is a complex process that is still not fully understood. … establishing good working relationshipsWebDec 1, 2007 · WEHI-231 B cell lines, classified as immature B cells [18], were maintained in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS) (HyClone, … firebase to mysqlWebWEHI-231 cells undergo apoptosis in response to antigen receptor cross-linking with anti-Ig reagents. Death is specifically triggered via surface IgM (sIgM); cross-linking sIgD, Ia or … establishing ground rules for teamsWebSep 1, 2007 · Thus, phenotypes could in principle be characterized systematically by comparing the proteomes of primary cells using unbiased proteomics approaches based on MS. Several analytical strategies exists that use MS for relative quantification of proteins and proteomes. ... The WEHI-231 B cell line was cultured in RPMI 1640 medium … firebase todoWebWEHI-231 cells in various phases of the cell cycle were isolated by centrifugal elutriation, and their response was evaluated following treatment with either anti-IgM or … establishing ground rulesWebB-cell line WEHI-231. These cells induce DNA-ladder formation and nuclear fragmentation by activating CAD during cytotoxic drug-inducedapoptosis.Moreover ... firebase tools cli