Phleomycin plates
WebbPhleomycin-resistant transformants are selected in Low Salt LB agar medium (yeast extract 5 g/L, Tryptone 10 g/L, NaCl 5 g/L, Agar 15 g/L, pH7.5) supplemented with 5 … Webb5-alpha Competent E. coli cells were transformed with 2 µl of the reaction sample following the Gibson Assembly Transformation Protocol from NEB. Next, 100 µl of the cells were plated onto LB medium plates with Phleomycin at a concentration of 20 ug/ml. The plates were incubated overnight at 37 °C. Figure 9.
Phleomycin plates
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Webb15 mars 2002 · Selection for phleomycin resistance was performed on YPD plates containing 7.5 µg/ml phleomycin (PHLEL0100; Cayla, France) (dissolve in water and add … WebbThe ble marker . The ble gene originates from the tallysomycin-producing actinomycetes species Streptoalloteichus hindustanus and encodes a small (13.7 kDa) protein …
Webb29 mars 2012 · Verification of mutant strains by drug sensitivity. (a) Phleomycin plates were incubated for 3 days at 30°C for S. cerevisiae and 5 days at 23°C for S. bayanus. Webb2 dec. 2010 · PDF Sectoring patterns of mutants in the rad52 epistasis group. Top panels: 5ORIΔ-ΔR chromoductants of rad50Δ, rad51Δ, rad52Δ, rad54Δ, rad55Δ, rad57Δ,... Find, …
Webb2 dec. 2010 · PDF Sectoring patterns of mutants in the rad52 epistasis group. Top panels: 5ORIΔ-ΔR chromoductants of rad50Δ, rad51Δ, rad52Δ, rad54Δ, rad55Δ, rad57Δ,... Find, read and cite all the ... Webb26 nov. 2007 · Twice as many colonies were observed when transformants were plated on MLS plus 50 μg/ml of phleomycin (left 2 plates) versus 100 μg/ml of phleomycin (right 2 …
WebbPhleomycin is mutagenic by introducing double-strand breaks in DNA. The ble gene of Streptoalloteychus hindustanus, which confers resistance to this substance, is widely …
Webbhygromycin B and phleomycin were purchased from Cayla. For efficient selection, some batches of hygromycin B required higher concentrations (up to 400 µg/ml). Phleomycin … bjbchatWebbAfter transformation incubate culture in YPD at 30 C for 6 hours to overnight, then chill on ice for 1 hour. Plate on YPD (pH 7) containing 10 mg/L phleomycin. Phleomycin is pH … dates that social security is paidWebb21 jan. 2003 · The population of strains from phleomycin plates displayed a significant increase in mean lovastatin yield (P < 0.001 in a bootstrap confidence interval for … bjb auctions stokeWebb1 jan. 2002 · 1. (A) Two-step gene disruption. An altered form of the target gene (e.g., carrying a single base mutation) present on a circular plasmid with a selectable gene (e.g., URA3) is integrated into the yeast genome by homologous recombination, selecting for inheritance of the selectable gene (e.g., Ura+). This generates a duplication of the target ... bjb chinakjcxdb.comWebbStore plates and unused medium at +4°C in the dark. Plates and medium containing Zeocin™ are stable for 1-2 weeks. Recommendation Invitrogen™ imMedia™ is available … bj baby bop riff whatWebb29 dec. 2008 · After mixing, regenerated protoplasts were spread on square plates containing 40 ml of solidified MM. Regeneration medium contained no antibiotic, 25 μg of phleomycin ml −1, or 10 μg of nourseothricin ml −1. Selection plates were incubated at 30°C for 5 days. Isolation of genomic DNA and RNA. dates that estimated taxes are dueWebb1 jan. 2012 · Invert plates after 30 min and place at 37°C (hygromycin); or RT overnight, followed by 30°C (phleomycin, see Note 12). 3. After 24–72 h, ensure the viability of the protoplasts and the validity of the transformation by inspecting, respectively, the “no-drug” control plates, and the positive and negative control plates. 4. b. j. baliga power semiconductor devices pdf